Human endogenous retrovirus-H insertion screening
نویسندگان
چکیده
منابع مشابه
Human endogenous retrovirus-H insertion screening.
Endogenous retroviruses (ERVs) and ERV-like sequences comprise 8% of the human genome. We aimed to analyze genome integration polymorphisms of human endogenous retrovirus (HERV)-H by the inter-retrotransposon amplified polymorphism (IRAP) technique using the sequences of LTR7A (450 bp), LTR7B (445 bp) and LTR7C (471 bp). Blood samples from 20 individuals (10 females and 10 males) of diverse eth...
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One of the most salient features of the replication strategy used by retroviruses is the transcription of the retroviral (RNA) genome into DNA followed by integration of this DNA product into the host cell genome. The integrated viral DNA copy, termed "provirus", can then serve as a template for the synthesis of further infectious virus particles. Stably integrated proviruses have been found to...
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The human genome represents a fossil record of ancient retroviruses that once replicated in the ancestors of contemporary humans. Indeed, approximately 8% of human DNA is composed of sequences that are recognizably retroviral. Despite occasional reports associating human endogenous retrovirus (HERV) expression with human disease, almost all HERV genomes contain obviously inactivating mutations,...
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We have demonstrated previously that the envelope proteins of a murine retrovirus (Moloney murine leukaemia virus) and a simian retrovirus (Mason-Pfizer monkey virus) have immunosuppressive properties in vivo. This property was manifested by the ability of the proteins, when expressed by tumour cells normally rejected by engrafted mice, to allow the envelope-expressing cells to escape immune re...
متن کاملSequence variability, gene structure, and expression of full-length human endogenous retrovirus H.
Recently, we identified and classified 926 human endogenous retrovirus H (HERV-H)-like proviruses in the human genome. In this paper, we used the information to, in silico, reconstruct a putative ancestral HERV-H. A calculated consensus sequence was nearly open in all genes. A few manual adjustments resulted in a putative 9-kb HERV-H provirus with open reading frames (ORFs) in gag, pro, pol, an...
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ژورنال
عنوان ژورنال: Molecular Medicine Reports
سال: 2013
ISSN: 1791-2997,1791-3004
DOI: 10.3892/mmr.2013.1295